To better assist you, could you please provide the following details:
Did you shut down the device the previous day?
Did you quit the Enpass app the previous day?
Additionally, please perform the following steps:
Disable the "Remember Keyfile" option.
Re-enable the "Remember Keyfile" option.
Lock Enpass.
Please enter the master password and keyfile to unlock Enpass and check if it works.
If it still doesn't work, kindly share the pathway of the keyfile you are using (to check if there are any special characters that might be causing the issue).
Lastly, please share a screenshot of the locked screen once the issue occurs.